| |
Enter
the concentration (in μg/μl)
of
the DNA used to transform: |
μg/μl |
| |
Enter
the volume (in μl) of
DNA added to the transformation
mix: |
μl |
| |
Enter
the volume (in μl) of
the total transformation reaction*:
|
μl |
| |
Enter
the volume (in μl) actually
plated
from the transformation mix
(for a single plate): |
μl |
| |
Enter
the number of colonies or
transformants from the plate
above: |
|
|
| Transformation Efficiency: |
|
Transformants / μg DNA
or |
|
Transformants / ng of DNA |
| |
| *This calculation assumes that
no futher dilutions of the
transformation reaction are
made after DNA, competent
cells, and media are added. |
| |
|
|
