Pick two types of tissue culture
vessels from the menus and the tool
will calculate the surface area
ratio. For a vessel not on the list,
choose "Custom Vessel: Enter parameters
manually". The surface area in cm2
can then be entered manually. After
the surface area ratio is calculated,
the desired cell dilution factor
can be entered manually to generate
the final dilution factor used to
split your cells.
calculated final dilution factor takes
into account both the desired cell dilution
factor and the difference in surface area
of the source and target tissue culture
vessels. Use this to dilute cells lifted
from one vessel into another vessel. Surface
areas given are of the total growth area
available to cells and, in the case of
a multi-well vessel, for a single well.
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