Mutate for Digest

Mutate for Digest accepts a DNA sequence as input and searches for regions that can easily be mutated to create a restriction site of interest. The program also displays protein translations so that you can see which reading frames are altered by the proposed mutations. Use Mutate for Digest to find sequences that can be converted to a useful restriction site using PCR or site-directed mutagenesis.

Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 10000 characters.

>CDS1
atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag
ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa
agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt
gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat
ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt
ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat
tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac
tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa
caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa
tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa
ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat
cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc
tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca
caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg
acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt
gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg
tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt
cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat
ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac
aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt
gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc
aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt
tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac
gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat
gacaatgaatag

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