| |
Enter the
concentration (in μg/μl) of
the DNA used to
transform: |
μg/μl |
| |
Enter the
volume (in μl) of
DNA added to the
transformation mix: |
μl |
| |
Enter the
volume (in μl) of
the total transformation
reaction*: |
μl |
| |
Enter the
volume (in μl) actually plated
from the
transformation mix (for a single plate):
|
μl |
| |
Enter the
number of colonies or
transformants from the
plate above: |
|
|
| Transformation
Efficiency: |
|
Transformants / μg DNA or |
|
Transformants / ng of DNA |
| |
| *This calculation assumes that
no futher dilutions of the transformation reaction
are made after DNA, competent cells, and media are
added. |
| |
|
|